乙型流感病毒抗原檢測試劑盒（免疫層析法）

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乙型流感病毒抗原檢測試劑盒（免疫層析法）

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3，產生組織切片非特異性染色
1）抗體孵育時間過長、抗體濃度高易增加背景著色。這可通過縮短一抗/二抗孵育時間、稀釋抗體來控制。這是zui重要的一條；
2）一抗用多克隆抗體易出現非特異性著色，建議試用單克隆抗體看看；
3）內源性過氧化物酶和生物素在肝臟、腎臟等組織含量很高（含血細胞多的組織），需要通過延長滅活時間和增加滅活劑濃度來降低背景染色；
4）非特異性組分與抗體結合，這需要通過延長二抗來源的動物免疫血清封閉時間和適當增加濃度來加強封閉效果；
5）DAB孵育時間過長或濃度過高；
6）PBS沖洗不充分，殘留抗體結果增強著色，在一抗、二抗或SP孵育之后的浸洗尤為重要；
7）標本染色過程中經常出現干片，這容易增強非特異性著色。
4，免疫組化染色呈陰性結果
1）抗體濃度和質量問題以及抗體來源選擇錯誤；
2）抗原修復不全，對于甲醛固定的組織必須用充分抗原修復來打開抗原表位，以利于與抗體結合;建議微波修復用高火4次*6min試試。有人做過實驗，這是*的時間和次數。若不行，還可高壓修復；
3）組織切片本身這種抗原含量低；
4）血清封閉時間過長；
5）DAB孵育時間過短；
6）細胞通透不全，抗體未能充分進入胞內參與反應；
7）開始做免疫組化，我建議你一定要首先做個陽性對照片，排除抗體等外的方法問題。

3, resulting in non-specific tissue section staining
1) antibody incubation time is too long, high antibody concentration easily increase background coloring. This can be controlled by reducing the primary antibody / secondary antibody incubation time and diluting the antibody. This is the most important one
2) The first antibody polyclonal antibody prone to non-specific coloring, it is recommended to try monoclonal antibody to see;
3) endogenous peroxidase and biotin in the liver, kidneys and other tissues is very high (including blood cells and more tissue), need to extend the inactivation time and increase the concentration of inactivator to reduce the background staining;
4) non-specific components and antibodies, which need to extend the secondary antibody from the immune serum to close time and the appropriate increase in concentration to enhance the sealing effect;
5) DAB incubation time is too long or too high concentration;
6) Inadequate washing with PBS, enhanced staining with residual antibody results, and dipping after primary antibody, secondary antibody or SP incubation are particularly important;
7) Dry specimens often appear during specimen dyeing, which easily enhances nonspecific staining.
4, immunohistochemistry showed negative results
1) Antibody concentration and quality problems and wrong selection of antibody sources;
2) incomplete antigen repair, fixed tissue for formaldehyde must be fully antigen retrieval to open the antigen epitope, in order to facilitate the combination of antibodies; recommended microwave repair with high fire 4 * 6min try. Someone has done experiments, this is the best time and frequency. If not, but also high-pressure repair;
3) the tissue section itself is low in this antigen;
4) serum blocking time is too long;
5) DAB incubation time is too short;
6) cells are not fully permeable, the antibody failed to fully enter the cell involved in the reaction;
7) begin to do immunohistochemistry, I suggest that you must first be a positive pair of photos, excluding methods other than antibodies.