流感B病毒診斷試劑盒（快檢方法）

廣州健侖生物科技有限公司

廣州健侖長期供應各種流感檢測試劑，包括進口和國產的品牌，主要包括日本富士瑞必歐、日本生研、美國BD、美國NovaBios、美國binaxNOW、凱必利、廣州創侖等主流品牌。

流感B病毒診斷試劑盒（快檢方法）

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室

操作步驟
1. 標準品的稀釋與加樣：在酶標包被板上設標準品孔10孔，在*、第二孔中分別加標準品100μl，然后在*、第二孔中加標準品稀釋液50μl，混勻；然后從*孔、第二孔中各取100μl分別加到第三孔和第四孔，再在第三、第四孔分別加標準品稀釋液50μl，混勻；然后在第三孔和第四孔中先各取50μl棄掉，再各取50μl分別加到第五、第六孔中，再在第五、第六孔中分別加標準品稀釋液50ul，混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中，再在第七、第八孔中分別加標準品稀釋液50μl，混勻后從第七、第八孔中分別取50μl加到第九、第十孔中，再在第九第十孔分別加標準品稀釋液50μl，混勻后從第九第十孔中各取50μl棄掉。（稀釋后各孔加樣量都為50μl，濃度分別為36ng/L，24 ng/L ，12 ng/L，6ng/L，3 ng/L）。
2. 加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑，其余各步操作相同）、待測樣品孔。在酶標包被板上待測樣品孔中先加樣品稀釋液40μl，然后再加待測樣品10μl（樣品zui終稀釋度為5倍）。加樣將樣品加于酶標板孔底部，盡量不觸及孔壁，輕輕晃動混勻。
3. 溫育：用封板膜封板后置37℃溫育30分鐘。
4. 配液：將20倍濃縮洗滌液用蒸餾水20倍稀釋后備用。
5. 洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復5次，拍干。

Steps
1. Standard dilution and sample loading: Prepare standard wells 10 wells in the enzyme-coated plate, add 100 μl each to the first and second wells, and then add standards to the first and second wells Then add 100μl from the first well and the second well to the third well and the fourth well, add 50μl standard diluent to the third and the fourth wells, and mix well; Then take out 50μl in each of the third and fourth wells, discard 50μl each and add them to the fifth and the sixth wells, respectively, and then add 50μl standard diluent to the fifth and the sixth wells, respectively; After mixing, take 50μl from each of the fifth and sixth wells and add them to the seventh and the eighth wells respectively, then add 50μl standard diluent in the seventh and the eighth wells respectively, Eight holes were added 50μl added to the ninth and tenth holes, and then in the ninth tenth hole standard diluent were added 50μl, after mixing from the ninth tenth hole each take 50μl discarded. (Each well was diluted to 50 μl at a dilution of 36 ng / L, 24 ng / L, 12 ng / L, 6 ng / L, 3 ng / L).
2. Sample loading: Set blank holes (blank control wells without sample and enzyme reagent, the rest of the same operation), the sample hole. Add 40μl sample diluent to the sample well on the ELISA plate, then add 10μl test sample (5 times final sample dilution). Add samples will be added to the bottom of the ELISA plate hole, try not touch the hole wall, gently shaking to mix.
3. Incubation: Seal the plate with a sealing plate and incubate at 37 ° C for 30 minutes.
4. Dosage: 20 times concentrated cleaning solution diluted 20 times with distilled water reserve.
5. Wash: Carefully remove the sealing plate membrane, discard the liquid, dry it, fill each well with a washing solution, and let it stand for 30 seconds before it is discarded. Repeat 5 times and pat dry.